Heat 250ml deionized water to 60C in a 300ml beaker, stirring at 350RPM
Add 2.5g CuSO4*5H20, wait until dissolved
Add 4.6g EDTA disodium dihydrate, wait until dissolved
Add 0.84ml of 1% Triton-X 100 solution into water
Dissolve 5.8g NaOH in 30ml deionized water, stir until clear, then add to bath
Add 4ml Formaldehyde (37% HCHO)
Lower stirring to 100RPM and begin filtration with 0.5 micron syringe filter and peristaltic pump. No intentional oxygenation or bubbling. Filter return is below surface for minimal splashing